Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineage
Fig 5
R-clamp architecture of SIVcpz Nef proteins.
(A) HZ-1 cells were transfected with an AP-1-luciferase reporter alone (Control) or together with wild-type or the indicated mutants of Nef from SIV strains from Pan troglodytes troglodytes (MB897, Cam5, and EK505) or Pan troglodytes schweinfurthii (Nok5 and Tan2). Luciferase activity in the transfected lysates was analyzed as in Fig 1C. The data shown are derived from three independent experiments, with SE indicated by error bars. The amino acid combinations at positions 83 and 120 are indicated as single-letter symbols and color-coded as explained in Fig 4. (B) CD4 downregulation by Nef in stably transduced Jurkat T cells was examined as in Fig 1C. Original dot plots are shown in S3 Fig.