Transient viral replication during analytical treatment interruptions in SIV infected macaques can alter the rebound-competent viral reservoir
Fig 2
Barcode size in pre-therapy plasma is predictive of detection and relative abundance in PBMC CA-vDNA.
(A) barcodes were partitioned into 0.5-log10 intervals based on their pre-ART peak plasma SIV RNA viral loads. The grey bars depict the number of barcodes in each viral load category, and the colored bars highlight the number of lineages that were observed in CA-vDNA in at least one PBMC sample during suppressed viremia between 172 and 313 dpi. (B) The pre-ART plasma frequency attributable to each lineage is plotted against its estimated frequency in vDNA. The Pearson correlation between all lineages detected in at least two PBMC samples is indicated for each animal, with the linear regression line shown in black. The dashed grey line represents a theoretical one-to-one ratio of barcode proportions. The color of the points indicates the number of PBMC samples each barcode was detected in. The number of PBMC samples analyzed was 6 for H860, 8 for H814, 6 for H34G and 5 for DFR6. For H814, no single barcode was detected in all 8 samples.