Binding to DCAF1 distinguishes TASOR and SAMHD1 degradation by HIV-2 Vpx
Fig 2
TASOR PARP-like domain is involved in DCAF1 binding.
(A) Schematic representation of HA-tagged TASOR or Flag-tagged TASOR constructs. 1–1670: TASOR long isoform. 1–1512: TASOR short isoform. 1–931: N-terminal fragment of TASOR. 630–1512: C-terminal fragment of TASOR short isoform. WT: TASOR (short isoform). ΔPARP: TASOR (short isoform) deleted of its PARP-like domain (106–319 aa). (B) Indicated HA-TASOR constructions were expressed in HeLa cells, then an anti-HA immunoprecipitation was performed. (C) Flag-Vpx WT and indicated HA-TASOR constructs were co-expressed in HeLa cells, then an anti-Flag immunoprecipitation was performed. (D) TASOR-Flag WT or TASOR-ΔPARP-Flag were co-expressed with HA-Vpx WT in HeLa cells, then an anti-Flag immunoprecipitation was performed. In each panel, the indicated proteins were revealed by western blot. For this figure, each shown immunoblot is representative of at least 3 independent experiments.