Resistance to lethal ectromelia virus infection requires Type I interferon receptor in natural killer cells and monocytes but not in adaptive immune or parenchymal cells
Fig 5
NK cells require IFNAR for optimal maturation, activation, and cytolytic killing.
(A) Graphs and concatenated flow cytometry histograms depicting GzmB and Prf expression in gated NK cells (NK1.1+ TCRβ-) from spleens of the indicated naïve or infected mice at six dpi. (B-C) Concatenated flow cytometry plots (B) and frequency (C) of GzmB+ Prf+ NK cells (NK1.1+ TCRβ-) in spleens of the indicated mice at 6 dpi. (D) Mice were infected with 3000 pfu of WT ECTV in the footpad and CFSE-labeled WT + Tap1-/- splenocytes at a 1:1 ratio were transferred i.v. Two hours before organ harvest at 6 dpi. Created with BioRender.com. (E-F) Concatenated flow cytometry plots (E) and proportions (F) of the preferential killing of Tap1-/- cells (CFSEhigh CD45.2) over WT cells (CFSEhigh B6.CD45.1) normalized to donor transfer ratio in spleens of the indicated naïve and infected mice at 6 dpi. Data are represented as mean ± SEM from two pooled independent experiments (N = 6 or 7 for each group, ANOVA with Tukey correction compared to Ifnar1fl/fl infected mice).