Herpes simplex virus 2 (HSV-2) evolves faster in cell culture than HSV-1 by generating greater genetic diversity
Fig 2
Genetic diversity in viral populations from original stocks and five times plaque-isolated HSV-1 and HSV-2 clones.
(A) Total number of MVs observed in each original stock, at a frequency equals or above a 1% limit of detection (see Material and Methods for details). The total number of MVs (y-axis) is separated into variant type and genomic location (x-axis). Variant type distinguishes between SNPs and InDels, discriminating nonsynonymous SNPs. The genomic location of each variant is categorized as non-coding or coding region. (B) Histograms show the number of MVs in each frequency range for each original stock. The frequency of each variant was examined and grouped in shown ranges (e.g., 10% to <20% frequency, 20% to <30% frequency and so on). SNPs and InDels were combined for this analysis. (C) Total number of MVs observed in each HSV-1 (left, blue bars) and HSV-2 (right, red bars) clones after five rounds of plaque-isolation, categorized by nonconservative changes (SNPs and InDels). See Sheets C and D in S1 Table for full lists of MVs position and frequency data. (D) Total number of variants detected in each clone are grouped by subtype and graphed showing mean +/- SEM (ns = not significant p > 0.05 by two-tailed Mann–Whitney U-test).