A plasmid locus associated with Klebsiella clinical infections encodes a microbiome-dependent gut fitness factor
Fig 5
Exogenous treatment of mice with SCFAs results in a terC fitness defect.
Seven days prior to inoculation, male and female C57BL6/J mice sourced from barriers RB16 and RB07 were treated with a SCFA cocktail or regular drinking water (sham). NTUH-K2044 and the isogenic ΔterC mutant (clone Kp2257) were mixed 1:1 and approximately 5x106 CFU were orally gavaged into mice (n = 19 per group). A fresh fecal pellet was collected daily from each animal, CFUs were enumerated, and log competitive indices (mutant:WT) were calculated (median and IQR displayed, *P < 0.05, **P < 0.005, ***P < 0.0005, one-sample t test compared to a hypothetical value of 0 or Holm-Sidak multiple-comparison test following one-way ANOVA).