Herpes Simplex Virus type 1 infects Langerhans cells and the novel epidermal dendritic cell, Epi-cDC2s, via different entry pathways
Fig 5
LCs and Epi-cDC2s both express HSV-1 entry receptors HVEM and nectin-1.
Epidermal MNPs were isolated from human abdominal or foreskin epidermis and labelled for flow cytometry as in Fig 2, as well as with antibodies to HVEM or nectin-1. The surface expression of HVEM and nectin-1 on LCs (purple histograms) and Epi-cDC2s (orange histograms) is shown in a representative donor. Mean receptor expression (gMFI) is graphed for LCs (purple) and Epi-cDC2s (orange). Circles indicate abdominal skin, triangles indicate foreskin. Two-tailed paired t-tests were used to compare the gMFI of HVEM or nectin-1 expression in LC and Epi-cDC2s, n = 3.