Fatty acid oxidation participates in resistance to nutrient-depleted environments in the insect stages of Trypanosoma cruzi
Fig 5
Flow cytometry reveals distinct patterns in fatty acid pools during epimastigote growth.
The epimastigotes were treated with 1 μM of BODIPY C1-C12 (500/510) and analysed by flow cytometry and fluorescence microscopy. A) 0 h. B) 24 h. C) 48 h. In the flow cytometry histograms, dashed peaks represent unstained parasites. Green-filled peaks represent stained parasites. D) Mean fluorescence per cell. The fluorescence for each cell was calculated using ImageJ software. All the experiments were performed in triplicates. Statistical analysis was performed with one-way ANOVA followed by Tukey’s post-test p < 0.05 using the GraphPad Prism 8.0.2 software program. We represent the level of statistical significance in this figure as follows: *** p value < 0.001; ** p value < 0.01; and * p value < 0.05. For p value > 0.05, we consider the differences not significant (ns).