Classical complement and inflammasome activation converge in CD14highCD16- monocytes in HIV associated TB-immune reconstitution inflammatory syndrome
Fig 5
Association between complement MAC deposition and inflammasome overactivation on monocytes during IRIS event.
(A) Overlay of monocyte subsets clusters from HLADR+DUMP-CD14+ and CD16+ population (“Total monocytes”) of TB-IRIS (n = 3) and TB non-IRIS (n = 3) patients on top of the t-SNE map based on CD14, CD16, C9 and FLICA expression. (A–right panel) Representative histograms of CD14, CD16, C9 and FLICA expression levels in each monocyte subset presented in the t-SNE plot. (B) Individual t-SNE maps for each group of samples (TB-IRIS and TB non-IRIS) based on expression levels of C9, FLICA, CD14 and CD16. Circles are drawn to highlight cell populations discussed in the text. (C) C9 and (D) FLICA expression levels (MFIs) were calculated as fold change over their respective experimental HCs for TB-IRIS (n = 10), TB non-IRIS (n = 9) and Non-TB, non-IRIS patients (n = 7), post-ART, within the distinct indicated monocytes subsets. Data are presented as median with interquartile range. *P < 0.05, **P < 0.01, when Mann-Whitney test was applied. Spearman correlations between FLICA fold change levels (E) or plasma levels of IL-18 (F) and C9 fold change levels found on monocytes from TB-IRIS and TB non-IRIS patient samples.