EDP-938, a novel nucleoprotein inhibitor of respiratory syncytial virus, demonstrates potent antiviral activities in vitro and in a non-human primate model
Fig 5
EDP-938 prevents primary transcription of RSV mRNAs.
Following a synchronized infection with RSV-A2 (MOI of 3), DMSO or 1000 nM EDP-938 was added at 0 hpi. Immunofluorescence analysis of inclusion bodies at 8 hpi in the presence of DMSO (A) or EDP-938 (B). N protein was detected with an N specific monoclonal antibody (red) and cell nuclei were stained with DAPI (blue). (C) RT-qPCR analysis of RSV N gene expression between 0 and 6 hpi. Samples were quantified using the ΔΔCt method relative to the mean expression of housekeeping gene GAPDH. The relative expression of positive sensitive RNA is shown. Data are representative of two independent experiments.