Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation
Fig 5
Granulomas from Mbv-infected cattle contain a higher number of MNGCs than Mtb-infected cattle.
(A, left image) Histological Haematoxylin and Eosin (H&E) staining of granulomatous lesions in thoracic lymph nodes of cattle challenged for 10 weeks with Mtb H37Rv strains. Scale bar, 200 μm. (A, right image) higher magnification of multinucleated giant cells (white square). Scale bar, 50 μm. (B, left image) H&E staining of granulomatous lesions in thoracic lymph nodes of cattle challenged for 6 weeks with Mbv AF2122/97. Scale bar, 200 μm. (B, right image) higher magnification of multinucleated giant cells (white square). Scale bar, 50 μm. (A and B) Granulomas are classified from early stage of maturation (type I and II) to late granulomas (type III and IV) (C) Quantification of the number of granulomas counted for each condition. (D) Quantification of bacterial load in each granuloma based on acid fast staining of the tissue section and expressed as the mean Acid Fast Bacilli (AFB) ± SEM. (E) Quantification of the number of MNGCs per granuloma ± SEM.