A swine arterivirus deubiquitinase stabilizes two major envelope proteins and promotes production of viral progeny
Fig 5
Nsp2TF antagonizes polyubiquitination of GP5 and M protein.
HEK-293T cells were co-transfected with plasmids expressing HA-Ub, M protein (A and C) or GP5 (B and D) and a plasmid expressing nsp2TF or the PLP2 catalytic sites mutant (nsp2TF-C/H>A). Cell lysates were collected at 48 h post transfection. IP was performed with anti-M pAb (A and C) or anti-GP5 pAb (B and D) and the membrane was probed with anti-HA mAb (A and B), a pAb recognizing anti-K48-linked polyubiquitin (C and D), or a viral protein-specific antibody. IP: immunoprecipitation; IB: immunoblotting; WCL: whole cell lysates; Ev: empty vector.