Therapeutic targeting of measles virus polymerase with ERDRP-0519 suppresses all RNA synthesis activity
Fig 4
Effects of ERDRP-0519 on single nucleotide addition.
A-C) Purified recombinant P-L complexes were incubated with the specified NTPs and a 25-nt RNA template driving both de novo initiation and back-priming [40] (A). A representative autoradiogram (n = 3) is shown, divided for clarity into dose-dependent inhibition of de novo RNA synthesis initiation at the promoter by ERDRP-0519 (B) and inhibition of 3’-elongation after back-priming (C). Products of less than 5-nt are not perceptible due to background from unincorporated 32P-labelled nucleotides. D) Dose-dependent inhibition of primer extension by ERDRP-0519 as in Fig 3B, but only GTP was added to visualize incorporation of the first nucleotide. EC50 values represent 4-parameter variable slope regression models, 95% confidence intervals are shown (n = 3).