m6A RNA methylation facilitates pre-mRNA 3’-end formation and is essential for viability of Toxoplasma gondii
Fig 2
Components of Toxoplasma m6A writer complex.
A) Immunofluorescence assay of endogenously HA-tagged WTAP, METTL3, and METTL14 subunits of the writer complex. Each protein is localized to the parasites’ nuclei. The outlines of parasites are shown in the merge panel for reference. B) Western blot of endogenously HA-tagged writer complex members. C) Relative quantification of writer complex member expression as assessed by Western blot densitometry. Data are presented as average ± standard deviation normalized to WTAPHA signal. * represents p ≤ 0.05 as assessed by Student’s t-test assuming unequal variances. D) Schematic summarizing the Toxoplasma writer complex membership as identified by co-immunoprecipitation experiments outlined in Table 2.