A high-affinity RBD-targeting nanobody improves fusion partner’s potency against SARS-CoV-2
Fig 5
SR31 could pair with RBM nanobodies to bind RBD.
(A, B) SR31 does not interfere with MR17 (A) or MR6 (B) for RBD-binding. In A, an RBD-coated sensor was pre-saturated in 200 nM of SR31 before incubating with SR31 alone (black) or a mixture (blue) of SR31 and MR17/MR6. In B, the sensor was saturated with MR6 before analyzed with SR31. For control purposes, the binding between RBD and the sybody used in the pre-incubation was also characterized (red). (C) Alignment of the biparatopic sybody (MR17-SR31)-RBD structure (MR17, grey; SR31, light blue; RBD, red) with the MR17-RBD structure (RBD, green) (PDB ID 7c8w) [35].