The CDT of Helicobacter hepaticus induces pro-survival autophagy and nucleoplasmic reticulum formation concentrating the RNA binding proteins UNR/CSDE1 and P62/SQSTM1
Fig 5
Effect of Helicobacter hepaticus CdtB expression on nucleus remodeling.
Wide field image of Hep3B transgenic cells expressing the CdtB of H. hepaticus strain 3B1 processed for the nuclear lamina (red) and P62/SQSTM1 fluorescent staining (green), and DAPI to counterstain the nuclei (blue). Yellow arrows indicate DAPI-lacking nucleoplasmic reticulum enclosing P62/SQSTM1 bodies. Boxes correspond to enlargement. Scale bar, 10 μm. Abbreviations: DAPI, 4′, 6′-diamidino-2-phenylindol; P62, P62/SQSTM1.