Complexome profile of Toxoplasma gondii mitochondria identifies divergent subunits of respiratory chain complexes including new subunits of cytochrome bc1 complex
Fig 5
Loss of the new complex III subunits affects parasite growth and mitochondrial membrane potential.
(A) Plaque assays of the parental line (parental) and four promoter replacement lines (rQCR8/9/11/12 / Rieske-HA). Parasites were grown for 9–10 days in the absence (-ATc) or presence of ATc (+ATc) before being fixed and stained with crystal violet. (B) Mitochondrial membrane potential detected using the JC-1 probe of parental (parental) and each of the four promoter replacement lines (rQCR8/9/11/12 / Rieske-HA) in the absence (blue) or presence (orange) of ATc, or valinomycin/atovaquone/antimycin a. Representative histograms of red fluorescence, which is dependent on mitochondrial membrane potential, recorded by flow cytometry are shown.