Multimerization- and glycosylation-dependent receptor binding of SARS-CoV-2 spike proteins
Fig 5
Binding of SARS-CoV-RBD proteins to serial Syrian hamster tissue slides.
SARS-COV-RBD proteins (50μg/ml) were applied to Syrian hamster lung tissue slides and detected using additional anti-Streptag and goat-anti-mouse antibodies sequentially. Where indicated SARS-CoV-RBD proteins were pre-incubated with recombinant ACE2 protein. Scalebar is 100μM. For the quantification, the intensity of gray pixels of stained Syrian Hamster lung tissue slides was measured with ImageJ version 1.52p. * indicates P<0.05 as determined by an unpaired two-tailed students T-test in the GraphPad software.