Intracellular niche-specific profiling reveals transcriptional adaptations required for the cytosolic lifestyle of Salmonella enterica
Fig 2
(A) RNA-seq results displayed as a volcano plot. Statistical significance is represented on the y-axis and magnitude of change on the x-axis. Statistically significant mRNAs and sRNAs are shown in blue dots for “up vacuole” and red dots for “up cytosol” categories. mRNAs/sRNAs that did not pass statistical significance are indicated by grey dots. Select genes of interest are indicated. (B) Pathway enrichment analysis. Top panels indicate regulons generated from transcriptional profiling of key regulatory mutants, while bottom panels display enriched gene sets generated from KEGG, GO, or from RNA-seq profiling of S. Typhimurium under 22 infection-relevant in vitro growth conditions. All displayed enrichments are significant (adjusted p-value <0.05) according to Fisher’s exact test with Benjamini-Hochberg correction for multiple comparisons. The number of genes in each category is shown in log2 scale. Only a selection of non-redundant pathways is presented; the complete list is available as S2 Dataset.