Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion
Fig 5
Cellular T and B responses are reduced in 4get/KN2 offspring from infected mothers >60 days post-primary immunization.
4get/KN2 28–35 days old were immunized in the hind footpad as described in the Materials and Methods. Draining popliteal lymph nodes were collected >60 days post primary immunization (A) GC B cells (CD19+GL-7+FAS+) frequencies (B) TFH(PD-1+CXCR5+, gated from CD4+) frequencies, (C) Th2 responses (GFP+IL-7R +/-) (D) Flow cytometry analysis of CD21/35 cell count (E) FDC (CD21/35+FDC-M2+CD45-) frequency. Flow data is concatenated representative of n n>4 mice per group and two independent experiments. Statistical analysis was calculated with Student’s t-test.