Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion
Fig 2
iNKT cells are the main cellular source of IL-4 in peripheral lymph nodes at steady state, and maternal S. mansoni infection reduces their secretion of IL-4.
Gating strategy in popliteal lymph nodes for invariant NKT cell (αGalCer+TCRαβ+ DX-5+), αβ T cells (TCRβ+CD3+), γδT cells (TCRβ-CD3+), NK T cells (TCRβ+DX-5+), NK cells (TCRβ-DX-5+) with respective staining control (unloaded tetramer) analyzed by flow cytometry in (A) pups from uninfected mother and (B) pups from infected mother at 28–35 days of age. Flow plots are concatenated from >4 mice per group with two biologically independent experiments.