Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation
Fig 7
Sensitivity of lytic induced LANA ring-like structures to LLPS.
A. iSLK cells were induced with Dox+NaB for 24h and then untreated or treated with 3.5% 1,6-HD for 10 min followed by IF for RFP-LANA (red), DAXX (green) or DAPI (blue). Scale bar = 10 μm. B. Quantitation of DAXX or LANA foci for experiments represented by panel A. N = 200 cells, **** p < .001, ** p < .01 two-tailed student t-test. C. Same as in panel A, but IF for RFP-LANA (red) and DAPI (blue). Scale bar = 10 μm. D. Quantification of IF images represented by panel A for % of cells with LANA ring-like structures. N = 200 cells, ****p < .001, two-tailed student t-test.