Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation
Fig 6
Redistribution of DAXX from LANA-NB during lytic induction.
A-B. iSLK RFP-LANA cells untreated (A) or induced by Dox+NaB for 24 h (B) were imaged with KSHV-DNA FISH (green) or DAXX IF (red) and DAPI (blue). Scale bar = 10 μm. C-D. Confocal images and 3D reconstructions for iSLK RFP-LANA cells untreated (C) or induced by Dox+NaB (D) for 24h were imaged with KSHV DNA FISH (green), LANA IF (red) and DAPI (blue). Scale bar = 5 μm. E-F. Confocal images and 3D reconstructions of iSLK RFP-LANA cells untreated (E) or induced by Dox+NaB (F) for 24h were imaged with KSHV DNA FISH (green), DAXX IF (red) and DAPI (blue). Scale bar = 5 μm.