Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation
Fig 5
Dynamic changes in LANA-NB morphology after lytic induction.
A. iSLK RFP-LANA cells untreated or induced by Dox or Dox+NaB for 24 h, or JQ1for 32 h were assayed by IF for RFP-LANA (red) and DAPI (blue). Scale bar = 10 μm. B. KSHV DNA FISH (green) combined with RFP-LANA IF (red) and DAPI (blue) in iSLK RFP-LANA cells untreated (top) or induced with Dox+NaB for 24h (lower panels). Scale bar = 10 μm. White arrows indicate examples of LANA ring-like structures.