Multiple roles of PP2A binding motif in hepatitis B virus core linker and PP2A in regulating core phosphorylation state and viral replication
Fig 6
Effect of linker mutations on virion secretion.
HepG2 were co-transfected with indicated HBc expression constructs and the HBV genomic construct defective in HBc expression and the culture supernatant was collected seven days post-transfection. A. Concentrated culture supernatant was resolved by agarose gel electrophoresis and transferred to nitrocellulose membrane. HBV DNA in virions and naked capsids were detected by using the HBV DNA probe. B. DNA virion secretion efficiency was determined by normalizing levels of virion DNA to cytoplasmic RC DNA levels (as shown in Fig 5). The efficiency from WT was set to 1.0. V, virion; Ca, capsid. **, P<0.01;***, P<0.001.