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EBV miRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escape

Fig 5

EBV miRNAs and their targets have strong inverse correlations in primary BLs and GCs.

For each CLASH miRNA-mRNA hybrid, mRNA and miRNA expression levels (c.p.m.) were correlated across all EBV-positive BL and GC tumors. Correlations were performed on hybrids that met the following criteria: 1. Average miRNA expression in tumors > = 10 c.p.m., 2. Average mRNA expression in tumors > 5 t.p.m., 3. > 3, and 4. miRNA-mRNA interactions occurred within the 3’-UTR, where miRNA mediated repression is more often effective[108]. Cumulative distribution plots were generated using ranked Spearman correlation coefficients for each species. As a control, expression levels of the miRNA of each hybrid pair analyzed was correlated with expression levels of a random mRNA across the same tumors. For each randomized interaction, 100 permutations were run. Correlation values of EBV miRNA-mRNA pairs were compared to randomized controls; P-values were generated using the KS test.

doi: https://doi.org/10.1371/journal.ppat.1009217.g005