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EBV miRNAs are potent effectors of tumor cell transcriptome remodeling in promoting immune escape

Fig 2

EBV miRNAs are over-represented in RNA induced silencing complexes (RISCs).

CLASH and miRNA-sequencing were performed in triplicate for SNU719 and Akata cells. (A-B, left) The viral percentage of total miRNA expression in each sample, (yellow triangles), and the percent of all miRNA-mRNA hybrids containing a viral miRNA, (green triangles), were calculated. The indicated P-values were calculated using unpaired Student’s t-tests. (A-B, right) The average number of miRNA-mRNA hybrids formed for each miRNA was normalized to its baseline expression level, . These mRNA-bound proportions were plotted in order of rank on the corresponding x-axis. Each circle represents an individual miRNA; circle size represents expression level; red circles indicate viral miRNAs. The indicated P-values were calculated using the Kolmogorov–Smirnov test (KS), comparing viral and human miRNAs.

doi: https://doi.org/10.1371/journal.ppat.1009217.g002