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Multiple blood feeding in mosquitoes shortens the Plasmodium falciparum incubation period and increases malaria transmission potential

Fig 2

A second blood meal accelerates oocyst development.

(A–B) Immunofluorescence assay of oocysts from control females (A) fed once (one red circle) or (B) fed twice (two red circles) at 8 d pIBM. (C) Oocyst from a female fed twice showing the release of mature sporozoites at 10 d pIBM. Sporozoites are labelled with circumsporozoite protein CSP (magenta) and DNA is stained with DAPI (gray). In all panels, scale bar = 10 μm.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1009131.g002