Impact of vaginal microbiome communities on HIV antiretroviral-based pre-exposure prophylaxis (PrEP) drug metabolism
Fig 5
Intracellular active metabolite formation after 24 hours grouped by Lactobacillus dominance or non-dominance.
(A) Amount of intracellular TFV-DP formed post-TFV administration after 24 hours at 37C in Lactobacillus dominant versus non-Lactobacillus dominant CVLs. Orange, greater than 50% dominance; Purple, less than 50% dominance. (B) Amount of intracellular TFV-DP formed post-TAF administration after 24 hours at 37C in Lactobacillus dominant versus non-Lactobacillus dominant CVLs. Orange, greater than 50% dominance; Purple, less than 50% dominance. (C) Amount of intracellular DPV formed post-DPV administration after 24 hours at 37C in Lactobacillus dominant versus non-Lactobacillus dominant CVLs. Orange, greater than 50% dominance; Purple, less than 50% dominance. (D) Rate of TFV-DP formation (amount/hour) vs. %Lactobacillus in CVLs after 24-hour incubation with TFV. Rate of degradation calculated as amount formed/24 hours. (E) Rate of TFV-DP formation (amount/hour) vs. %Lactobacillus in CVLs after 24-hour incubation with TAF. Rate of degradation calculated as amount formed/24 hours. (F) Rate of DPV formation (amount/hour) vs. %Lactobacillus in CVLs after 24-hour incubation with DPV. Rate of degradation calculated as amount formed/24 hours.