Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion
Fig 5
Calcineurin phosphatase activity remains functional in VZV infected cells.
(A) Fluorescence microscopy images of MeWo cells transiently expressing GFP-NFATC1 at 16 hpi with pOka-TK-RFP that were untreated (medium) or treated with DMSO or pimecrolimus (Pim, 10 μM) 30 min prior to ionomycin stimulation (Iono, 1 μM; 30 min); pOka-TK-RFP (red), GFP-NFATC1 (green), and nuclei stained with Hoechst 33342 (blue), and a composite image. Scale bars = 15 μm. (B) Box and whisker plots of GFP-NFATC1 localization in VZV infected cells. Of the total GFP-NFATC1 expressing cells that were also infected with TK-RFP, the percentage of cells with GFP-NAFTC1 translocated to nucleus (N), diffused in nucleus and cytosol (N/C), or localized in cytosol (C). Boxes represent 25–75 percentile, whiskers extend to 10–90 percentile, the median is the horizontal band, and the mean (+) of cells (n = 12 fields of view, 41–66 cell per field of view) from three independent experiment. Dots are outliers. Statistical difference were analyzed by two-way ANOVA for each localization group (N, N/C, and C) compared to that in the untreated, shown are the significantly different pairs (****, p < 0.0001).