CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreatitis
Fig 5
CVB3 VP1 could suppress CAK activity in vivo.
(A) Time-course analysis of the accumulation of phosphorylated/nonphosphorylated CDK2 and CDK4 by indicated antibodies. Original blots are shown in S22 Fig for statistical analysis. (B) The expression level and phosphorylation of RNA Pol II CTD were detected by antibodies against RNA Pol II CTD and phospho-RNA Pol II CTD. CVB3 infection was the positive control. Original blots are shown in S23 Fig for statistical analysis. (C) Immunoblot analysis of the accumulation of phosphorylated/nonphosphorylated pRb with phospho-pRb Ser 780, 795, 807/811 antibodies. CVB3 infection was the positive control. Original blots are shown in S24 Fig for statistical analysis. (D) The abundance of RNA Pol II CTD, phospho-RNA Pol II CTD and CDK7 were analyzed by Western blot, and this assay was performed at 48 hours after transfecting various plasmids. Original blots are shown in S25 Fig for statistical analysis. (E) Western blotting analysis of the expression of phosphorylated/non-phosphorylated pRb and CDK7 with phospho-pRb Ser 780, 795, 807/811 and CDK7 antibodies. Original blots are shown in S26 Fig for statistical analysis.