E2 ubiquitin-conjugating enzyme UBE2L6 promotes Senecavirus A proliferation by stabilizing the viral RNA polymerase
Fig 5
(A) Immunoprecipitation and Western blot of BHK-21 cells cotransfected with Flag-tagged SVA proteins and HA-tagged UBE2L6; proteins immunoprecipitated with rabbit anti-HA. (B) Western blot showing that the UBE2L6-3D interaction is specific. p3D-Flag and pUBE2L6-HA cotransfected cell lysates were collected, and then co-IP was performed using mouse anti-Flag. (C) Colocalization of UBE2L6 and viral 3D. Confocal microscopy of BHK-21 cells transfected with pUBE2L6-HA then infected with SVA (MOI 0.01) for 16 h. SVA 3D (green), pUBE2L6-HA (red), and nuclei (blue). Experiments were performed in triplicate.