BH3-only sensors Bad, Noxa and Puma are Key Regulators of Tacaribe virus-induced Apoptosis
Fig 9
Regulation of apoptotic factors during TCRV and JUNV infection.
(A) Protein levels of selected apoptotic factors. Infection with either TCRV or JUNV was performed at an MOI of 0.1 in Vero76 cells and cell lysates were harvested from 1–4 dpi. Samples were subjected to Western blot and stained for full-length caspase (Casp) 3 and 9, their cleavage products (cCasp), p53, Puma, Noxa, Bad and Bad-P (Ser112). NP staining served as a positive control for infection and staining for Vinculin was used as a loading control. Western blots were evaluated by measuring pixel intensities with normalization to the loading control. Quantifications of data obtained 4 dpi are shown as mean values and standard deviations of at least two independent experiments. Statistical significance was determined using two-way ANOVA (*p≤0.05, **p≤0.01, ns not significant). (B) CPE formation in Vero76 cells during TCRV and JUNV infection. Bright field images of mock and infected cells were taken 4 dpi and are shown with 100 μm scale bars.