Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion F
Fig 5
RSB1 and CR9501 target similar epitopes with different angles of approach and an “induced fit”.
Three views, each rotated by 45 degrees for A) DS-Cav1-RSB1 complex and B) CR9501 complex. For simplicity, only one protomer is shown bound by one Fab. CR9501 is colored dark green for the heavy chain and light green for the light chain. RSB1 coloring matches Fig 1. C-D) Open-book views of the RSB1 (C) and CR9501 (D) interfaces showing total buried surface areas for their respective epitopes and paratopes. E-F) HCDR3 for RSB1 (E) and CR9501 (F) are shown as cartoons, while F1 and F2 are depicted as surface. Residues Leu100 (RSB1) and Ile100A (CR9501) are shown with sticks. Tyr98 on CR9501 which causes an induced fit is also shown as sticks.