Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion F
Fig 2
X-ray structure of the DS-Cav1-RSB1 complex.
A) The structure of the DS-Cav1-RSB1 complex is depicted with surfaces colored in light and dark gray for two DS-Cav1 protomers, and orange and gold for the F2 and F1 subunits of the third protomer. Fab RSB1 is depicted with pink and magenta surfaces for the L and H chains, respectively. B) Zoomed view of the region boxed in A), after a rotation of ~90 degrees around the y axis. RSB1 CDR loops are depicted as cartoons and labelled. The total RSB1 epitope surface is colored cyan. C) Same view as in B) with the epitope hydrogen bonding residues on DS-Cav1 colored purple and the residues making van der Waals contacts colored green. RSB1 CDR residues making contacts with the purple and green regions are shown as sticks. D) Zoomed view of the RSB1 epitope on DS-Cav1, to highlight salt bridge interactions (sticks and dashes) only. For clarity, this view is slightly re-oriented with respect to panels B and C. E) Rotated and zoomed view of panel C highlighting RSB1 cross-protomer interactions with DS-Cav1 (dashes).