Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor
Fig 2
2D-gel electrophoresis highlights spots differentially abundant between priming conditions.
Left panels: two-dimensional difference gel electrophoresis (2D-DiGE) with the three fluorescent channels corresponding to the three CyDyes (Cy5, Cy3 and Cy2) merged. Right panels: 2D-SDS-PAGE stained by mass spectrometry-compatible silver staining protocol are shown for eggs sampled in ovaries (top gels) or sampled within 16 h post-laying (bottom gels). Eggs were collected 3 days after female priming. Spots significantly (p < 0.05, Mann-Whitney test) 1.5 differentially abundant in eggs from B. thuringiensis compared to control, S. entomophila compared to control, both B. thuringiensis and S. entomophila compared to control and B. thuringiensis compared to S. entomophila are represented by green, red, yellow and purple circles, respectively. The same color code is used in Table 2 presenting a summary of proteins identified. A full list of the proteins identified and associated statistics is available in S3 Table. Six biological replicates per condition, each containing 70–74 eggs, for a total of 433 eggs from 117 different females were included in the 2D-DiGE analysis.