Eukaryotic translation initiation factor 5A and its posttranslational modifications play an important role in proliferation and potentially in differentiation of the human enteric protozoan parasite Entamoeba histolytica
Fig 6
Cellular distribution of EhDHS and EheIF5A isoforms and post translational modification of EheIF5A1/2.
(A) Cellular fractionation of EhDHS1, EhDHS2, EheIF5A1, and EheIF5A2. Trophozoites of the transformants expressing HA-tagged EhDHS1, EhDHS2, EheIF5A1, and EheIF5A2 were fractionated as described in Materials and Methods, and subjected to immunoblot analysis using anti-HA monoclonal antibody, anti-hypusine, anti-CPBF1, and anti-CS1 polyclonal antisera. CPBF1 and CS1 served as control of organelle and cytosolic proteins, respectively. (B) PTMs of HA tagged EheIF5A. Approximately 30 μg of total lysates from the transformants expressing HA-tagged EheIF5A1 and EheIF5A2 were subjected to SDS-PAGE under reducing conditions and immunoblot analysis using anti-hypusine antibody.