Eukaryotic translation initiation factor 5A and its posttranslational modifications play an important role in proliferation and potentially in differentiation of the human enteric protozoan parasite Entamoeba histolytica
Fig 4
Determination of the size of the complex of EhDHS1 and EhDHS2.
(A) Purified Recombinant EhDHS1 and 2 were co-expressed, copurified, and were subjected to 4–16% BN-PAGE. After electrophoresis the gel was destained. Native page marker (Invitrogen) was used as a protein standard. (B) Immunoblot analysis following BN-PAGE of co-expressed N-terminal His-tagged EhDHS1 and C-terminal S-tagged EhDHS2, using anti-His and anti-S tag antibodies respectively.