Eukaryotic translation initiation factor 5A and its posttranslational modifications play an important role in proliferation and potentially in differentiation of the human enteric protozoan parasite Entamoeba histolytica
Fig 3
Characterization of wild type, mutant and co-expressed forms of EhDHS1 and EhDHS2 (A) Schematic representation of DHS1 and DHS2 mutant. Amino acid residues mutated are shown in grey. Amino acid positions are also shown. (B) Enzymatic activity of recombinant EhDHS1, EhDHS2, EhDHS1_K295F, and EhDHS2_F302K using EheIF5As substrates. The mean ± S.D. of three independent experiments performed in triplicate are shown. (C) Immunoblot analysis of co-expression of EhDHS1, which contains the amino-terminal His-tag, and EhDHS2, which contains the carboxyl-terminal S-tag, using anti-His and anti-S tag antibodies. (D) Specific activity of co-expressed recombinant EhDHS1 and EhDHS2 using EheIF5A1 and EheIF5A2 as substrates. The means ± standard deviations of three independent experiments performed in triplicate are shown.