Identification and characterisation of serotonin signalling in the potato cyst nematode Globodera pallida reveals new targets for crop protection
Fig 5
The role of TPH-1 in host plant invasion behaviour.
A. G. pallida J2s were soaked in either water (control) or water with the TPH inhibitor CPA (10 mM) for 24 h. This was followed by the addition of either serotonin (10 mM) or fluoxetine (2 mM) for 30 min and stylet thrusting was scored for 1 min. Data are mean ± s.e.m. n = 27 for serotonin and n = 40 for fluoxetine. *** p<0.001; Two way ANOVA with Bonferroni's multiple comparisons. B. CPA impaired the ability of J2s in root invasion. J2s were collected at 24 h post hatching and pre-incubated for 24 h in water without (control), or with the addition of CPA (100 μM). J2s were applied to individual potato hairy root cultures at 3 infection points with 25 J2s per infection point. 13 days later roots were stained with acid fuchsin and nematodes visualised. Data are mean ± s.e.m. for 19 plants; *** p<0.001 unpaired Student's t-test.