Actin and an unconventional myosin motor, TgMyoF, control the organization and dynamics of the endomembrane network in Toxoplasma gondii
Fig 6
TgMyoF is required for Rab6 vesicle transport.
(A-B) Left panel: TgMyoF-AID parasites expressing EmGFP-Rab6 treated with EtOH (A) or IAA (B) for 18 hours before imaging. Dashed oval indicates the PV surrounding a 2-parasite vacuole. Location of the nucleus is indicated by the blue circles. Area in the yellow box was used to make inset (middle panel). Middle panel: Images of the Rab6 compartment taken at 5 second intervals. Right panel: Kymograph depicting Rab6 vesicle motion. (C) Run frequency (# of directed runs/parasite/minute) of Rab6 vesicles in control and TgMyoF depleted parasites. Combined results from two independent experiments. Mean from each independent experiment is indicated with large circles. These values were used to calculate the average (horizontal bar), standard error of the mean (error bars), and P value. Raw data is shown with smaller colored circles. Experiment 1 in magenta, experiment 2 in cyan. (D) Frequency distribution of Rab6 vesicle velocities in RH parasites (orange) and in TgMyoF-AID parasites treated with EtOH (blue) or IAA (green) for 18 hours.