Actin and an unconventional myosin motor, TgMyoF, control the organization and dynamics of the endomembrane network in Toxoplasma gondii
Fig 5
TgMyoF knockdown results in fragmentation and dispersion of post-Golgi compartments.
(A) TgMyoF-AID parasites expressing Neon-Rab5, EmGFP-Rab6, Neon-Rab7, GFP-Syn6, GFP-DrpB (magenta), or untransfected parasites, were treated with EtOH (left panels) or IAA (right panels) for 18 hours before fixation. Parasites were stained with anti-IMC1 to highlight the parasite periphery (yellow) and DAPI to stain DNA (cyan). Untransfected parasites were stained with anti-SORTLR antibody (magenta; lower panel). Scale bar is 5μm. (B) For each post-Golgi compartment, the number of objects was quantified in control and TgMyoF knockdown parasites. Combined results from two or three independent experiments. Mean from each independent experiment is indicated with large circles. These values were used to calculate the average (horizontal bar), standard error of the mean (error bars), and P value. Raw data is shown with smaller colored circles. Experiment 1 in magenta, experiment 2 in cyan, experiment 3 in grey.