Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia
Fig 2
Morphology of the Tilapia brain cells (TiB) and cytopathic effect (CPE) induction on TiB induced by TiPV and transmission electron micrographs of the TiPV-infected TiB cells.
(A) The TiB cells at passage 1, 10 days; (B) The TiB cells at passage 2, 3 days; (C) TiB cells infected with TiPV at passage 3 at 5 days post infection; (D) TiB cells infected with TiPV at passage 6 at 3 days post infection (Bar = 100 μm). (E) Virus particles existed in the cytoplasm and nuclei (white arrow), Nu: nucleus.(Bar, 1 μm); (F) High magnification of the region in the white rectangular box indicated in Panel A, virus particles aggregated in the cytoplasm (Bar, 200 nm); (G) The virus releasing at the plasma membrane of the TiB cell. (Bar, 200 nm); (H) Purified TiPV particles negatively stained with 2% phosphotungstic acid (Bar, 200nm).