Evolving MRSA: High-level β-lactam resistance in Staphylococcus aureus is associated with RNA Polymerase alterations and fine tuning of gene expression
Fig 5
Differential gene expression in SH1000 follows the acquisition of chromosomal mecA and the rpoB/C mutations.
Two-set proportional Venn diagram illustrating the total number of differentially expressed genes (DEGs) altered in A) lysA::pmecA (SJF4996) (grey) and lysA::pmecA rpoB-H929Q (SJF5003) (red), B) lysA::pmecA (SJF4996) (grey) and lysA::pmecA rpoC-G740R (SJF5034) (blue) and C) lysA::pmecA (SJF4996) (grey) and lysA::kan rpoB-H929Q (SJF5010) (green) compared to WT (white background). D) Three-set proportional Venn diagram displaying the shared DEGs among lysA::pmecA (SJF4996) (grey), lysA::pmecA rpoB-H929Q (SJF5003) (red) and lysA::pmecA rpoC-G740R (SJF5034) (blue) compared to WT (white background). 3 common DEGs (mecA, lysA, nirR) are marked by a circle showing reduced expression. E) The lysA gene (marked by a circle) was shared among four strains based on comparisons from c) and d) compared to WT (white background). The expression of lysA was reduced in all four strains compared WT, marked by a downward arrow.