A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization
Fig 6
Chitin-induced polymerization of Mg1LysM homodimers.
(A) Model inferred from the crystal structure of Mg1LysM in which a continuous structure of Mg1LysM homodimers and chitin is formed. Alternating chitin molecules (in grey sticks) and Mg1LysM homodimers (in red and green), each of them with two chitin-binding sites, are shown. (B) Dynamic light scattering (DLS) heat maps of Mg1LysM, C. fulvum Ecp6 and RiSLM treated with chitohexaose in molar ratios of 1:0, 1:2 and 1:5 (protein: chitohexaose), respectively. The particle size distribution is indicated as a color scale ranging from blue (lowest amount) to red (highest amount) for a particle size range of 1 nm to 100 um.