A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization
Fig 2
The chitin binding groove formed by two Mg1LysM protomers.
(A) A chitin trimer (GlcNAc)3, displayed as grey sticks, was identified in a binding pocket formed by two Mg1LysM protomers (indicated in yellow and red, respectively). (B) Representation of the binding pocket from the top. (C) Detail of the chitin binding site. The amino acids involved in direct chitin trimer binding (26GDTLT30 and 56NRI58) are represented with blue sticks and labelled. In addition, K31 and D54 (represented in green) of the two different Mg1LysM protomers form a salt bridge that tightly closes the binding pocket. Grey discontinuous lines represent the salt bridge and the hydrogen bonds between the protomers.