Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production
Fig 7
Analysis of nascent HBV RNAs following SRSF10 KD or 1C8 treatment.
(A) dHepaRG cells were transfected with siRNA against SRSF10 and then infected with HBV (MOI of 250 vge/cell). Edu labelling was performed at D7pi for 2 hours. (B) dHepaRG cells were infected with HBV and then treated three times with 1C8 (40μM) at D7, D9 and D11pi. EU incorporation was performed at D13pi for 2 hours. (C) and (D) Run-on analyses. Intracellular RNA was extracted from transfected/treated cell cells and either directly quantified using HBV primers (Total HBV RNAs) or purified using the Click-iT Nascent RNA Capture kit to quantify newly synthetized RNAs (nascent HBV RNAs). Control was provided by treating cells with Actinomycin D (ActD at 10mg/ml) added to cells 20 min before labeling (see Methods). <LOD: under the limit of detection.