Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production
Fig 6
Combined effect of sRSF10 KD and 1C8 treatment on HBV-infected dHepaRG cells.
(A) Outline of the experimental procedure: dHepaRG cells were transfected once or twice with siRNA targeting SRSF10, then infected with HBV (MOI of 250 vge/cell), and 7 days later treated three times with 1C8 (10μM). (B) Western blot validation showing SRSF10 depletion. (C) Quantification of intracellular HBV RNAs. Results are expressed as the mean normalized ratio +/- SD between treated and/or siSRSF10 transfected cells and siCTL-transfected cells of 3 independent experiments, each performed in triplicate.