Fluorescent protein tagging of adenoviral proteins pV and pIX reveals ‘late virion accumulation compartment’
Fig 2
pV-mCherry and pIX-mCherry display a large intranuclear compartment late in infection.
(A) Infection of A549 cells with HAdV5 pV-mCherry at 24 hpi and 48 hpi. (B) Infection of A549 cells with HAdV5 pIX-mCherry at 24 hpi and 48 hpi. The cells were imaged by live-cell confocal laser-scanning fluorescence microscopy. A representative cell is shown for each condition. The dsDNA signal is represented by Hoechst 33342 stain (Hoechst). The nuclear lamina is represented by a GFP-nanobody recognizing lamin A (Lamin A). pV and pIX localization is detected through the viral pV-mCherry and pIX-mCherry fusion construct (pV-mCherry/pIX-mCherry). The signal overlap is represented in color (merge). Nuclear regions of interest are enlarged (zoom) with colored corners indicating the channel color. Scalebars indicate 10 μm.