Rapid in vitro generation of bona fide exhausted CD8+ T cells is accompanied by Tcf7 promotor methylation
Fig 5
Repeat peptide stimulation induces a distinct pattern of transcription factor expression.
Transcription factors (TF) were analyzed in OT-I CD8+ T cells on day 5 of culture. Cells either unstimulated (no peptide), stimulated one time (single peptide) or daily (repeat peptide) shown. (A) Representative flow cytometry plots of the frequency of TF-expressing live CD8+ T cells shown for the differently treated cultures. (B) Pooled data depicting the MFI of TCF1 and TOX in live CD8+ T cells. (C) Pooled data showing the frequency of EOMES and T-bet expressing live CD8+ T cells. Each symbol represents one animal (n = 9–12), with n = 7–9 independent experiments performed. Line depicts mean ± SE. ANOVA with Tukey’s post hoc test was performed on TCF1 MFI, Student’s t test with Welch’s correction was used for TOX MFI and Wilcoxon signed rank test for data in (C). *<0.05, **P<0.01, ***P<0.001, ****P<0.0001.